《植物生理学报》 2016, 52(1): 55-61

9个常绿杂交冬青的抗寒能力比较

曾雯, 金晓玲^*, 邢文, 胡曼筠

中南林业科技大学风景园林学院, 长沙410004

通信作者：金晓玲；E-mail: 121191638@qq.com

摘　要：

以9个常绿杂交冬青品种的离体叶片为供试材料进行低温胁迫处理, 用电导法配以Logistic方程计算出半致死温度(LT₅₀), 并测定了超氧化物歧化酶(SOD)、丙二醛(MDA)、脯氨酸和可溶性蛋白各项指标, 运用隶属函数法进一步分析其抗寒能力。结果表明, 杂交冬青的相对电导率(Rec)随着温度的降低而上升, LT₅₀均在–34.91~–14.13 °C之间; 低温胁迫下, 随着温度的降低, MDA含量逐渐上升, SOD活性和可溶性蛋白含量呈先上升后下降的趋势, 而脯氨酸含量呈先升后降或上升的变化趋势。综合LT₅₀和各指标隶属函数分析法得出抗寒性强弱顺序为: ‘Shamrock’>‘Forsteri’>‘Schworbel’s Compacta’>‘Anna’s Choice’>‘Tensaw’>‘Blue Maid’>‘Bronze Beauty’>‘Oscar Gray’>‘Savannah’。

关键词：杂交冬青; 抗寒性; 半致死温度; 隶属函数法

收稿：2015-10-08   修定：2016-01-11

资助：林业公益性行业科研专项经费项目(201404710)和湖南省“十二五”重点学科(风景园林学) (湘教发[2011]76号)。

Comparison of cold resistance among nine evergreen Ilex cultivars

ZENG Wen, JIN Xiao-Ling^*, XING Wen, HU Man-Jun

College of Landscape Architecture, Central South University of Forestry and Technology, Changsha 410004, China

Corresponding author: JIN Xiao-Ling; E-mail: 121191638@qq.com

Abstract:

In order to evaluate the cold resistance of nine evergreen Ilex cultivars, the physiological mechanism of detached-leaf from nine evergreen Ilex cultivars under low temperature stress was studied. Their semi-lethal temperatures were calculated by relative electric conductivity (Rec) and logistic equation. Meanwhile, the contents of SOD, MDA, free proline and soluble protein were determined to provide their cold resistance. The results indicated that Rec increased as the droping of temperature, and LT₅₀ was between –14.13°C and –34.91°C. With the temperature decreased, the contents of MDA increased, and the SOD activity and soluble protein contents increased firstly, then decreased, and the contents of free proline increased or increased firstly, then decreased. Synthetic evaluation of LT₅₀ and the membership function indicated that the ranking of cold tolerance of Ilex cultivars was: ‘Shamrock’>‘Forsteri’ > ‘Schworbel’s Compacta’ > ‘Anna’s Choice’ > ‘Tensaw’ > ‘Blue Maid’ > ‘Bronze Beauty’ > ‘Oscar Gray’ > ‘Savannah’.

Key words: Ilex cultivars; cold resistance; semi-lethal temperature; the membership function